Effect of Conjugation with Polypeptide Carrier on the Enzymatic Degradation of Herpes Simplex Virus Glycoprotein D Derived Epitope Peptide

摘要

Two conjugates with epitope peptide ~(278)LLEDPVGTVA~(287)derived from glycoprotein D(gD-1)of Herpes simplex virus(HSV)were synmesized for analysis of the effect of conjugation on protection against enzymatic degradation.In this design,the turn-forming epitope core ~(281)DPVG~(284)was positioned in the central part of the peptide and elongated by three amino acids from the native sequence at both termini.Conjugation was achieved by me introduction of amide bond or thioether linkage between the C-terminal of the HSV peptide and the side chain of four lysine residues of the oligotuftsin derivative used as carrier molecule.We compared the proteolytic stability of the conjugates in diluted human sera as well as in rat liver lysosomal preparation.The data obtained in lysosomal preparation at two pH values(pH 3.5 and 5.0)show that the type of covalent bond between the carrier and the epitope peptide had no significant effect,as compared to the stability of the free,unconjugated peptide.Based on the identification of degradation fragments by mass spectrometry we found marked differences in the lengths and amounts of oligopeptides obtained.In contrast,in 10% and 50% human serum the conjugation provided full protection against enzymatic hydrolysis over 96 h,while the free peptide was decomposed quickly.