Applications of chamber devices with nonlinear optical image system

摘要

In this study, we use laser to fabricate the plastic chamber for animal experiments in vivo. Laser cutting plastics process offers to cut a complex shape at high speed with the absence of hot stress and deformation. We combine this novel method to fabricate the dorsal skinfold plastic chamber and the ear plastic chamber. The chamber was applied on the nude mice and combined with optical system to improve the time of observation and the position of accuracy. The plastic dorsal skinfold chamber (DSC) exhibited good properties than the metallic DSC. The metallic DSC only offered monitoring for more than seven days after implantation. In contrast with the metallic DSC, the plastic DSC enabled monitoring to be observed for more than 12 days after implantation. This plastic DSC and ear chamber are more applications on short-term and long-term mode of disease. These chamber techniques combined with nonlinear microscopy will have many applications in clinic. The nonlinear optical effects have advantages for monitoring the biological processes, such as cellular autofluorescences were excited by two photons excitation and collagen structures were observed by second harmonic generation. The chamber model combines with nonlinear optical microscopy to visualizing cells and tissue structures in vivo. This chamber model allows repeat observation of the same sites for long periods of time and investigation of cells and collagen structures interaction without histological staining and sacrificed animals.