Field studies of control of Anoplophora glabripennis (Coleoptera: Cerambycidae) using fiber bands containing the entomopathogenic fungi Metarhizium anisopliae and Beauveria brongniartii

摘要

The Asian longhorned beetle, Anoplophora glabripennis, was first found attacking urban street treesin the United States in 1996 and in Canada in 2003. This tree-killing invasive insect has long beena major pest in China and is difficult to control because immature stages live within wood andlong-lived adults are often located high in tree canopies. A microbial control product (BiolisaKamikiri) consisting of non-woven fiber bands impregnated with cultures of an entomopathogenic fungus, Beauveria brongniartii, is marketed in Japan for control of a congeneric orchard pest.Replicated field trials were conducted in Anhui, China to compare Biolisa Kamikiri with similarlyprepared bands containing Metarhizium anisopliae for control of A. glabripennis. One fungalband was placed at 2-2.5 m height, around the stem or major scaffold branch on each of 40 willowtrees (Salix spp.) per plot, with five plots for each fungal treatment and five control plots. Adultbeetles collected from fungal-treated plots 7-22 days after bands were attached to trees diedfaster than adults from control plots. Beetles exposed to B. brongniartii bands consistently diedfaster than controls throughout this period, while results from plots with M. anisopliae bandswere not as consistent in differing from controls. Numbers of adult beetles from plots of eachfungal species dying in 10 days were greater than controls (16% of beetles) but did not differbetween fungal treatments (34-35%). Oviposition in fungal-treated plots was approximatelyhalf that in control plots. Locations of adult beetles and oviposition scars within tree canopieswere quantified to determine optimal locations for band placement. Most adult beetles were found 3.5-m high in trees, with adults in B. brongniartii -treated plots higher within trees than adults inother plots.