Development and Validation of First Order UV-Spectrophotometric Method for Simultaneous Estimation of Tolperisone Hydrochloride and Diclofenac Sodium in Combined Tablet Dosage Forms

摘要

A simple, sensitive, precise and specific first order UV spectrophotometric method for simultaneous estimation of tolperisone hydrochloride and diclofenac sodium in bulk and combined tablet dosage form has been developed using distilled water as a solvent. The absorbances were measured at zero crossing point (ZCP) of tolperisone hydrochloride and diclofenac sodium at 261 nm and 276 ran, respectively. The linearity ranges for tolperisone hydrochloride and diclofenac sodium were 6-21 μg/ml and 2-7 μg/ml respectively. The accuracy of the method was assessed by recovery studies and was found to be 100.43 ± 0.22% and 100.48 ± 0.14% for tolperisone hydrochloride and diclofenac sodium respectively. Results of marketed formulation shows that this method can be applied successfully to marketed formulation. The method is simple, accurate and rapid; requiring no preliminary separation and can therefore be used for routine simultaneous analysis of both drugs.