Erratum: AID dysregulation in lupus-prone MRL/Fas lpr/lpr mice increases class switch DNA recombination and promotes interchromosomal c-Myc/IgH loci translocations: Modulation by HoxC4 (Autoimmunity (2011) 44: 8 (664))

摘要

Eleven of the 14 c-Myc/IgH junction sequences (TL01, TL02, TL6, TL10, TL13, TL10, TL30, TL38, TL42, TL158, and TL138-the second TL10, as italicized, was a mislabel of the sequence TL19) depicted in Figure 7 are correct. These 11 sequences were obtained by nested PCR using degenerate IgH switch region primers 5'-AGCTCATTCCAGCTCAGCTCAGCCT-3'' (first round PCR) and 5'-AGCTCAGCTCAGCCTARCCC-AGCTC-3' (second round PCR), rather than the 5'-TGAGGACCAGAGAGGGATAAAAGAGAA-3' (first round PCR) and 5'-CACCCTGCTATTTCCTTGTTGCTAC-3/ (second round PCR), as stated in Materials and methods. Only degenerate primers can anneal to DNA of different S regions, thereby allowing amplification of c-Myc/IgH junctions arising from IgH breakpoints at different S regions (Potter et al., Blood 97: 260-269, 1997; Kovalchuk et al., J. Exp. Med. 204: 2989-3001, 2007). The three remaining sequences, TL-125, TL-187, and TL-162, possibly resulted from a PCR artifact involving primers 5'-TGAGGACCAGAGAGGGATAAAAG-AGAA-3' (first round PCR) and 5'-CACCCTGCTATTTCCTTGTTGCTAC-3' (second round PCR), and, therefore, should be excluded from data analysis.