Hydrolytic Quinoa Protein and Cationic Lotus Root Starch-Based Micelles for Co-Delivery of Quercetin and Epigallo-catechin 3-Gallate in Ulcerative Colitis Treatment

摘要

The accumulation and sustained release of drugs in the colonic inflammatory region are the favorable strategy for treating ulcerative colitis(UC).In this study,we developed a synergistic anti-inflammatory drug(quercetin/EGCG)-loaded micelle using hydrolytic quinoa protein(HQP)and cationic lotus root starch(CLRS)by a layer-by-layer assembly method.The encapsulation efficiency of quercetin and EGCG in the Que-HQP-EGCG-CLRS micelles reached 91.5 and 89.4%,respectively.This composite micelle exhibited a core-shell structure,where Que-HQP-EGCG was the core and CLRS was the coating shell.Moreover,the in vitro experiments indicated that these micelles can make Que/EGCG pass through gastric environments stably and delay their release in the intestine.Animal experiments further confirmed that the Que-HQP-EGCG-CLRS micelles can efficiently accumulate in the colonic inflammatory region and enable sustained release of drugs(more than 24 h),thus notably alleviating the symptoms of UC.These results suggested that Que-HQP-EGCG-CLRS micelles have good gastric stability,colonic inflammatory-accumulated effect,and sustained drug release ability,which are a promising co-delivery system for UC treatment.