This aim of this study was to evaluate and compare the efficacy of different chemical methods to disinfect gutta-percha cones (GP). Eighty-six size 80 GP cones were used. The cones were contaminated by immersion in saliva and Enterococcus faecalis. Four chemical agents were used: 1% sodium hypochlorite (G1), 2% chlorhexidine gluconate (G2), 10% povidone iodine (G3) and 0.9% saline solution (G4). GP cones were immersed in the solutions for periods of 1 and 10min. After the disinfection procedure, the cones were incubated in blood heart infusion and the presence of bacterial growth was analysed by turbidity of the medium. In G4, bacterial growth was observed in all specimens; G3 showed growth after immersion for 1min when contaminated with E. faecalis; G1 showed diverse results after the immersion for 1min. Meanwhile, G1 and G3 after 10min, and G2 at both times evaluated did not show bacterial growth. The immersion of GP cones in 2% chlorhexidine gluconate for 1min was an effective method for GP disinfection, while 10% povidone iodine and 1% sodium hypochlorite needed 10min of immersion to disinfect the GP.
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