Abstract Background and Aims Whole bunch fermentation is widely used in red wine production but research on whole bunch fermentation is limited, especially for cool climate Pinot Noir. Inclusion of whole bunches or grape stems was investigated in Pinot Noir wine production with respect to extraction of phenolic compounds and aroma production. Methods and Results Five Pinot Noir wines were microvinified by including grape stems or whole bunches at various levels: destemmed grapes (DS), 100 stems added back (DS100), 30 whole bunches (WB30), 60 whole bunches (WB60) and 100 whole bunches (WB100). The DS100, WB60 and WB100 treatments showed significantly increased tannin and monomeric phenolics but decreased anthocyanin in wines, which would consequently influence the mouthfeel and colour of wine. Volatile compounds responsible for green/vegetative, spicy, woody and medicinal aromas, including 3‐isobutyl‐2‐methoxypyrazine, 3‐isopropyl‐2‐methoxypyrazine, eugenol, ethyl cinnamate and phenol, were significantly increased in DS100, WB60 and WB100 treatments. The WB30 treatment did not show a significant increase of methoxypyrazines in the resultant wine. Conclusions By adding stems or a high proportion of whole bunches in fermentation, increased extraction of tannins may improve the mouthfeel and structure of Pinot Noir wine, but the significantly increased concentration of methoxypyrazines could negatively affect wine quality due to the enhanced green characteristics. Significance of the Study This study reveals the significant impact of stem inclusion during fermentation on phenolic and aroma compounds in Pinot Noir wine, which provides insights into better use of whole bunches and stems to improve Pinot Noir wine quality.
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