A modified approach to identification of the sickle cell anemia mutation by means of allele‐specific polymerase chain reaction

摘要

AbstractThe allele‐specific PCR approach has been modified by introducing a second mismatch at the 3′‐penultimate link of the primer and used to identify the sickle cell anemia mutation (A → T transversiion in the sixth codon of the human β‐globin gene causing Glu → Val substitution in the protein), thus obviating the problem of an interpretationally ambiguous 3′‐terminal mismatch including T residue. © 199