ISOLATION, CHARACTERIZATION AND SCREENING OF ALKALINE PROTEASE PRODUCING ALKALIPHILIC BACTERIA FROM THE POLLUTED HABITATS

摘要

A large number of alkaliphilic bacteria producing alkaline protease were isolated from different habitats polluted with proteinaceous materials. After primary screening eight isolates SPD-9, PD34, AP-1, BP, LP-2, AT, SH2 and STLP were selected and characterized. Based on morphological and biochemical characteristics, all of them were identified as Bacillus species. Among the four reported media tested (M1213, M660, Horikoshi and Halophilic bacillus medium) M660 and Horikoshi medium supported maximumenzyme production. Among all strains LP2 showed maximum alkaline protease production followed by SH2 and PD34 strains.