Induction of systemic resistance in Lycopersicon esculentum cv. PKM1 (tomato) against Cucumber mosaic virus by UV-B irradiation on calli.

摘要

Studies were done to evaluate the effect of UV-B irradiation on calli to induce resistance in the regenerated Lycopersicon esculentum cv. PKM1 (tomato) plantlets against Cucumber mosaic virus (CMV)-induced diseases. Plantlets regenerated from the UV-B (T_L, T_M, T_H) treated calli are referred to as T_L, T_M and T_H plantlets respectively, and they hold remarkably increased soluble phenolics, salicylic acid and peroxidase content compared to control plantlets without any treatment. All the plantlets challenge-inoculated with CMV showed disease severity ranging from T_M=32%, T_H=56%, T_L=78% and C=92%. But in contrast, ELISA results show that CMV accumulation in young uninoculated leaves were significantly less in the T_H plantlets than T_M plantlets, which reveals that T_H treatment affects virus accumulation and also affected the plantlet's physiological properties and chlorophyll content due to high UV-B dosage. However, in T_M plantlets, physiological properties were not affected and these plantlets were effectively elicited against CMV attack. Exposure of UV-B (T_M) on calli certainly activates an effective defense response and empowers the tomato plantlet's survival against viral attack.