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High-speed spectral imager for imaging transient fluorescence phenomena

机译:高速光谱成像仪,用于成像瞬态荧光现象

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We describe fluorescence spectral imaging results with the microscope computad-tomography imaging spectrometer (mu CTIS). This imaging spectrometer is capable of recording spatial and spectral data simultaneously. Consequently, mu CTIS can be used to image dynamic phenomena. The results presented consist of proof-of-concept imaging results with static targets composed of 6-mu m fluorescing microspheres. Image data were collected with integration times of 18 ms, comparable with video-frame-rate integration times. Conversion of raw data acquired by the mu CTIS to spatial and spectral data requires postprocessing. The emission spectra were sampled at 10-nm intervals between 420 and 710 nm. The smallest spatial sampling interval presented is 1.7 mu m. (C) 1998 Optical Society of America. [References: 15]
机译:我们用显微镜计算机断层扫描成像光谱仪(mu CTIS)描述荧光光谱成像结果。该成像光谱仪能够同时记录空间和光谱数据。因此,μCTIS可用于对动态现象进行成像。呈现的结果包括概念验证的成像结果,其中包含由6微米荧光发微球组成的静态目标。图像数据的采集时间为18毫秒,与视频帧速率的积分时间相当。 mu CTIS采集的原始数据到空间和光谱数据的转换需要后处理。发射光谱以420至710 nm之间的10 nm间隔采样。呈现的最小空间采样间隔为1.7微米。 (C)1998年美国眼镜学会。 [参考:15]

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