Ex vivo evaluation of human fetal membrane closure.

摘要

BACKGROUND AND OBJECTIVE: This study compares stress and leak point pressure of human fetal membranes following closure with suture, laser welding, or a new tissue sealant, SynthaSeal. STUDY DESIGN/MATERIALS AND METHODS: Samples of fetal membrane were bisected and repaired with suture, laser welding with albumin solders, or a tissue sealant. Representative tissue samples were assessed by histology. Anastomotic strength was evaluated by tensiometry. Additionally, leak pressure was measured after membrane samples were placed onto an infusion pump/pressure transducer, incised, and repaired as above. RESULTS: Membrane stress was strongest (P<0.001) for the tissue sealant group. Sutured samples were stronger than laser-welded samples. Membrane leak pressure was greatest for the tissue sealant group. The suture group demonstrated a higher leak pressure than the laser-welded group. CONCLUSIONS: Human fetal membranes were not suited for repair with laser welding under the conditions tested. A new tissue sealant, SynthaSeal, may provide a simple, effective method for membrane closure following fetoscopy, amniocentesis, and open fetal surgery. Copyright 2002 Wiley-Liss, Inc.