Proteomic analysis of human T?cell‐derived exosomes reveals differential RAS/MAPK signaling

摘要

Abstract Exosomes are cell‐derived vesicles that have been implicated in the pathogenesis of many inflammatory diseases. More specifically, it has been shown that T?cell‐derived exosomes can induce immunological responses; however, little is known about the mechanism and the molecular content of these vesicles. Here, we used a proteomic approach to characterize human T?cell‐derived exosomes. We found that specific proteins of the RAS signaling pathway were enriched in exosomes derived from activated T?cells, and that these vesicles induced ERK phosphorylation in recipient immune cells. Our findings support a mechanistic role of exosomes in cellular activation, and further studies should consider exosomes as a biomarker for inflammatory diseases.