Frequency of protease and reverse transcriptase drug resistance mutations in naive HIV-infected patients.

摘要

BACKGROUND: Infections with drug-resistant HIV viruses in naive subjects may cause antiretroviral (ARV) treatment failure. The prevalence of ARV resistance mutations in HIV-1 transcripts of infected naive patients from northeast Mexico was determined in this study. METHODS: RNA was extracted from plasma samples of 42 naive individuals who were diagnosed between February 2001 and September 2003 as HIV-1 infected. Both protease (Pr) and reverse transcriptase (RT) were sequenced in 30 patients. In six samples only the RT segment was sequenced and in three samples only the protease segment was analyzed. RESULTS: One of 36 isolates (2.8%) had the M184V resistance mutation to nucleoside retrotranscriptase inhibitors. In the Pr segment, only minor mutations were detected in 27/33 isolates (81.8%). CONCLUSIONS: In this first study, prevalence of major mutations associated with ARV resistance in naive patients in northeast Mexico is low compared to other countries, perhaps due to a low level of exposure of thispopulation to ARV drugs.