Proof of the successful development of Nile tilapia (Oreochromis niloticus) clones by DNA fingerprinting.

摘要

Out of 6 potentially homozygous female Nile tilapia mitogynes (clone founders), 837 gynogenetic and 427 diploid control offspring were produced. To determine the inheritance of parental bands, a total of 150 putative gynogenetic and 35 diploid control offspring were screened by multilocus DNA fingerprinting, random amplified polymorphic DNA (RAPD) and simple sequence repeat-anchored PCR (SSRa-PCR). These techniques showed that clone founders as well as gynogenetic offspring were genetically homozygous. Multilocus DNA fingerprinting and RAPD demonstrated that carryover of male chromosomal DNA by the use of UV-irradiated sperm for induction of gynogenesis did not occur and that the clonal lines could be accurately distinguished from each other. In contrast to these methods, the primers used in SSRa-PCR did not have the power to determine the absence of paternal genomic transmission due to a lack of visible informative paternal bands.