首页> 外文期刊>Applied biochemistry and biotechnology, Part A. enzyme engineering and biotechnology >Enhancement of Enzymatic Hydrolysis and Klason Lignin Removal of Corn Stover Using Photocatalyst-Assisted Ammonia Pretreatment
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Enhancement of Enzymatic Hydrolysis and Klason Lignin Removal of Corn Stover Using Photocatalyst-Assisted Ammonia Pretreatment

机译:光催化辅助氨预处理增强玉米秸秆的酶解和克拉森木质素去除

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摘要

Photocatalyst-assisted ammonia pretreatment was explored to improve lignin removal of the lignocellulosic biomass for effective sugar conversion. Corn stover was treated with 5.0–12.5 wt.% ammonium hydroxide, two different photocatalysts (TiO_2 and ZnO) in the presence of molecular oxygen in a batch reactor at 60 °C. Various solid-to-liquid ratios (1:20–1:50) were also tested. Ammonia pretreatment assisted by TiO_2-catalyzed photo-degradation removed 70 % of Klason lignin under the optimum condition (12.5 % ammonium hydroxide, 60 °C, 24 h, solid/liquid?=?1:20, photocatalyst/biomass?=?1:10 with oxygen atmosphere). The enzymatic digestibilities of pretreated corn stover were 85 % for glucan and 75 % for xylan with NH_3-TiO_2-treated solid and 82 % for glucan and 77 % for xylan with NH_3-ZnO-treated solid with 15 filter paper units/g-glucan of cellulase and 30 cellobiase units/g-glucan of β-glucosidase, a 2–13 % improvement over ammonia pretreatment alone.
机译:探索了光催化剂辅助的氨预处理,以提高木质素去除木质纤维素生物质的效率,从而实现有效的糖转化。在60°C的间歇式反应器中,在分子氧存在下,用5.0–12.5 wt。%的氢氧化铵,两种不同的光催化剂(TiO_2和ZnO)处理玉米秸秆。还测试了各种固液比(1:20-1:50)。在最佳条件下(12.5%氢氧化铵,60°C,24小时,固/液?=?1:20,光催化剂/生物质?=?1)(在12.5%的氢氧化铵下),在TiO_2催化光降解的辅助下氨预处理可去除70%的Klason木质素。在氧气气氛下为:10)。预处理的玉米秸秆的酶消化率分别为:葡聚糖为85%,含有NH_3-TiO_2处理的固体的木聚糖的酶消化率为75%,含有NH_3-ZnO处理的固体的15%滤纸/ g-葡聚糖的木聚糖的酶消化率为82%,木聚糖的酶消化率为77%。 β-葡萄糖苷酶的纤维素酶和30个纤维二糖酶单位/ g-葡聚糖的含量,比单独的氨预处理提高了2-13%。

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