Staining with highly sensitive Coomassie brilliant blue SeePico Stain after Flamingo fluorescent gel stain is useful for cancer proteomic analysis by means of two-dimensional gel electrophoresis.

摘要

Highly sensitive Coomassie brilliant blue SeePico Stain was applied for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS). After staining with Flamingo Fluorescent Gel Stain, the images of the protein spots were analyzed, and 424 protein spots were detected. After washing with Milli-Q water three times, the gels were re-stained with SeePico Stain and the images of the protein spots were analyzed; 272 spots were detected. To assess whether SeePico Stain alters MS analysis, a spot was picked up and was analyzed by LC-MS/MS. The MS analysis showed good protein identification. These results show a possible role for SeePico Stain in cancer proteomics using 2-DE and MS.