The use of RAPD and STS analyses for marking genes of homeologous group 5 chromosomes of common wheat.

摘要

The search for STS (sequence-tagged site) and RAPD (random amplified polymorphic DNA) markers tightly linked to some genes of homoeologous group 5 chromosomes of common wheat Triticum aestivum, more specifically, awns inhibitor genes (B1), vernalization response gene (Vrn1) and homoeologous chromosome pairing gene (Ph1), was conducted. To estimate the linkage of the gene with the marker, wheat lines marked with recessive alleles b1 and vrn1 were used. RFLP (restriction fragment length polymorphism)and SSR (simple sequence repeat) analyses of isogenic wheat lines were conducted to characterize the chromosomal region transferred to the isogenic line from the donor parent. In RAPD analysis of isogenic wheat lines marked with recessive alleles b1 andvrn1, 95 arbitrary primers were used. To develop STS markers, analysis of the primary structure of RFLP markers Xpsr426 and Xcdo504, tightly linked to the Vrn1 gene, and the Xpsr1201 marker, located at the Ph1 locus, was carried out. Two markers that are tightly linked to the Vrn1 gene (5AL) - RAPD marker Xr405 and STS marker Xsts426 - were obtained in this work. In addition, there is every reason to believe that Xsts426 can be used as a PCR marker of genes Vrn2 (5BL) and Vrn3 (5DL), while Xsts1201 isa marker of Ph1 (5BL).