Effect of penicillamine on acute phase protein, inflammationand tissue during acute nanoparticle silver toxicity in mice.

摘要

LD50 of intraperitoneal (IP)nanoparticled silver in 4 groups of 6 BALB/c mice was determined. Effect ofpenicillamine on nano particle toxicity in 6 groups of 6 female BALB/c mice given 50 or200 mg/kg concurrently, or 4 hours after was assessed. Six mice were used asuntreated controls. Haptoglobin (Hp), serum amyloid A (SAA), tumor necrosis factoralpha (TNF-α), Interferon gamma (IFN-γ) and interleukin 6 (IL-6) were assessed. Micegiven nanosilver showed moderate degeneration of hepatocytes with mononuclearcell infiltration and swelling, with interstitial nephritis, peribronchiolitis, bronchiolarassociated lymphatic tissue, interstitial pneumonia with macrophages andlymphocytes infiltration in lumen of alveoli. No lesions were observed in brain or heart.Severity of lesions appeared less in mice given penicillamine and no lesions were foundin controls. The LD50 for nanosilver was 1500 PPM and there were significant increasesin Hp, SAA, TNF-α, IFN-γ and IL-6 in all groups except those receiving 200 mg/kgpenicillamine.