Polymorphism in growth hormone gene of Khuzestan buffalo.

摘要

Authors report polymorphism in growth hormone gene by testing 200 Khuzestan buffalo blood samples selected by herd book and phenotypic standard for the breed. Restriction patterns were visualized by agarose gel electrophoresis and ethidium bromide staining. Individual PCR-RFLP fragment sizes for the gene were determined by visualizing band patterns under ultraviolet light. A 428 bp fragment of growth hormone gene spanning over 4th exon, 4th intron and 5th exon was amplified and digested with AluI restriction endonuclease to identify polymorphism at this locus. Digestion rendered 2 types of genotypes. Distribution of genotypes and allele frequency by Hardy-Weinberg equation were LL=194 (97%) and LV=6 (3%). Most buffalo were homozygous (LL) and less heterozygous for the valine allele compared with homozygous leucine allele. Results suggested that diversity of GH gene in Khuzestan buffalo was very low and showed no polymorphisms. Production traits could not be associated with buffalo growth hormone genotypes due to their monomorphic haplotype.