Effect of pyocin from Pseudomonas aeruginosa on liver and spleen pathology induced by Leishmania donovani in BALB/C mice

摘要

We describe liver and spleen pathology in mice induced by experimental infection with Leishmania donovani 30 or 60 days after immunization with or without pyocin from Pseudomonas aeruginosa. Pseudomonas were swabbed from human burn, wound and urine sampled patients. Four groups of 20 BALB/C mice each were administered 70ml/mg pyocin intraperitoneally (IP) and then challenged with or without (Leishmania controls) 1X107 L. donovani IP 30 and 60 days later. At 30 and 60 days, 2 mice from each group weresacrificed for liver and spleen microscopy. Gross pathology in L. donovani controls showed enlargement of liver and spleen with ascites. By microscopy, in mice given pyocin and infected 30 days later with L. donovani the main hepatic lesion was perivascular aggregation of mononuclear cells (MNC) but spleens appeared normal. In those infected 60 days later, the liver also had multiple MNCs in portal region mainly macrophages and lymphocytes, but with dilated edematous sinusoids and vacuolar degenerationof adjunct parenchyma. The spleen had focal polymorphic leukocytes with macrophage aggregation in sub-capsular region and hyperplasia of white pulp. The findings suggest that pyocin given IP by 30 days may lessen liver and spleen pathology caused by Leishmania donovani. Further study with higher, more frequent doses of pyocin may be warranted.