Isolation of pasteurella multocida serotypes A, B and E in marsh buffalo

摘要

Capsular Serotypes B and E of P. multocida can cause severe acute hemorrhagic septicemia (HS) in bovines. Authors describe pasteurella serogroups isolated from buffalo located in Thi-Qar, Basra and Misan marshes, Iraq between May 2017 and April 2018.Nasal swabs and blood were collected from 393 buffalo cultured on enriched selective media for identification of P. multocida by biochemical, gram stain and commercial API 20E system. Conventional PCR confirmed findings, genotypes and virulent genes. Wedetermined that 194 buffalo appeared clinically diseased with HS and 199 were normal. Pasteurella multocida in diseased buffalo was 34% significantly higher (P < 0.05) compared with 14% in healthy buffalo PPrevalence in buffalo located at marshes was 25.4% (100 out 393) from nasal swabs but only 1.03% in blood (2 out of 194). Records show that 35% were type A, 63%type B and only 2% type E being significantly different (P 0.05). PCR confirmed P. multocida yielding species-specific 460 bp with KMT1T7 andKMT1SP6 primers. Reference P. multocida serotype A, B and E with characteristic DNA laddering at 1,044 bp, 760 bp, and 511 bp, respectively. We find that P. multocida, genotypes A, B and E are endemic in diseased and healthy buffaloes located in marshesin Southern Iraq.