Characterization and transcriptional regulation of thioredoxin reductase 1 on exposure to oxidative stress inducing environmental pollutants in Chironomus riparius

摘要

We characterized thioredoxin reductase 1 (TrxR1) from Chironomus riparius (CrTrxR1) and studied its expression under oxidative stress. The full-length cDNA is 1820. bp long and contains an open reading frame (ORF) of 1488. bp. The deduced CrTrxR1 protein has 495 amino acids and a calculated molecular mass of 54.41. kDa and an isoelectric point of 6.15. There was a 71. bp 5' and a 261. bp 3' untranslated region with a polyadenylation signal site (AATAAA). Homologous alignments showed the presence of conserved catalytic domain Cys-Val-Asn-Val-Gly-Cys (CVNVGC), the C-terminal amino acids 'CCS' and conserved amino acids required in catalysis. The expression of CrTrxR1 is measured using quantitative real-time PCR after exposure to 50 and 100. mg/L of paraquat (PQ) and 2, 10 and 20. mg/L of cadmium chloride (Cd). CrTrxR1 mRNA was upregulated after PQ exposure at all conditions tested. The highest level of CrTrxR1 expression was observed after exposure to 10. mg/L of Cd for 24. h followed by 20. mg/L for 48. h. Significant downregulation of CrTrxR1 was observed after exposure to 10 and 20. mg/L of Cd for 72. h. This study shows that the CrTrxR1 could be potentially used as a biomarker of oxidative stress inducing environmental contaminants.