Protein Purification Workflow Development Using Bio-Rad's NGC~(TM) Chromatography System

摘要

High purity protein is a common requirement for biochemical and structural studies. Acormmon approach is to recombinantiy express an affinity-tagged version of the protein of interest. This is, however, not always a viable option. Here we discuss protein purification workflow development for untagged proteins and introduce a new indicator of method performance, the purity quotient difference (PQD).