A New Mixed-Ligand Coordination Polymer: Crystal Structure, Molecular Docking and Alleviating Effect on Myocarditis by Inhibiting the NLRP3-Caspase-1 Pathway in Cardiomyocytes

摘要

A new mixed-ligand coordination polymer {[Co(Hbidc)(2,2'-bpy) (H2O)2]·1.5H2O}n (1) has been successfully prepared by the reaction of Co(NO3)2·6H2O, 2, 2'-bipyridine (2,2'-bpy) as well as benzimidazole-5, 6-dicarboxylic acid (H3bidc) in the mixed solvent of water and DMA under solvothermal conditions. Furthermore, a mechanical grinding method was used to produce the nanostructure 1 with the average size of 263 nm. The integrity of H9 C2 cardiomyocytes cell membrane after nano 1 treatment was detected by LDH detection. After nano 1 incubation, the inflammatory level in the H9 C2 cardiomyocytes was evaluated by western blot and ELISA measurement. In addition, the ROS accumulation in H9 C2 cardiomyocytes after nano 1 treatment was determined by H2DCFDA detection assay. Finally, possible interactions between the studied complex 1 and protein has been probed using molecular docking simulation.