Participation of Yolk Proteins in Regulation of Germ Genes Expression from Intergeneric F1 Hybrids of Bream, Roach and Blue Bream

摘要

Analysis of connection between dynamics of yolk lipovitellin degradation and specific features of germ genes expression in early development of intergenetic reciprocal F1 hybrids of the bream, roach and blue bream was studied. It is a known fact that the main yolk protein in oocyte and embryo is lipovitellin, making reserve, nutritional and structural functions. But we think that lipovitellin is active component of internal fluid embryo invironment too in wich embryo cells and germ genes are developing and expressing. There is a view that synchronous expression of parental alleles of genetic loci from fish hybrids is related, as a rule, to kindred crossing, and asynchronous expression -to remote crossing. But when we analysed character of expression of some loci of intergeneric reciprocal Fl hybrids (6-Pgd, Ldh-B, beta-Est-1, 2, 3, Aat-1, Me-1, 2) with different expression time in embryogenesis, we show that character of loci expression is related to stage of development also. As objects we used zygotes, embryos, larvas and frys of bream, roach, blue bream and intergeneric reciprocal F1 hybrids. Identification and analysis of enzymes activity and lipoviteelline properties were performed using methods of disk-, gradient and SDS-electrophoresis in polyacrylamide gel. There are some arguments for regulation function of lipovitellin : first argument is connected with different expressions character (synchronous and asynchronous) of germ genes and with different distribution of isozymes activity in early and late stages of hybrid F1 embryogenesis; and second - with biocatalytic activity of lipovitelline because of oogenesis isoenzymes connected with lipovitelline by weak connection. When first locus expression was timed to the early stages (blastodisk - gastrula), the gene parental alleles were activated asynchronously according to the maternal types. When the first expression was timed to later stages (yolk sac resorption), parenteral alleles were activated synchrono...