An Efficient Fluorescent Probe for Tetracycline Detection Based on Histidine-Templated Copper Nanoclusters

摘要

In this contribution, we established a simple, green, fast and cost-effective strategy to prepare green-emitting fluorescence copper nanoclusters (Cu NCs) by using histidine as a stabilizer and ascorbic acid as a reducing agent. A series of character- ization methods including fluorescence spectroscopy, UV-vis absorption spectroscopy, fourier transform infrared spectro- scopy (FT-IR), transmission electron microscopy (TEM) and X-ray photoelectron spectroscopy (XPS) were used to characterize the Cu NCs. The histidine-capped Cu NCs (His-Cu NCs) was well dispersed and exhibited good water solubility, excellent photo- stability and salt resistance. Interestingly, it was discovered that tetracycline caused a sensitive and selective decline in the fluorescence intensity of His-Cu NCs for the first time. The proposed analysis system showed a wide linear range from 0.1 to 110 μM, and the detection limit was 0.047 μM. After that, the fluorescent assay was successfully employed to analyze tetracy- cline in lake water samples.