Effects of biochar on photosystem function and activities of protective enzymes in Pyrus ussuriensis Maxim. under drought stress

摘要

The mechanisms how orchard mulching biochar influenced the photosynthetic apparatus and the role of protective enzyme activities were employed to evaluate the value of biochar application for the cultivation of fruit trees in arid areas. Potted 2-year-old seedlings of Pyrus ussuriensis Maxim. were studied to determine the effects of orchard mulching with biochar on photosystem function, Rubisco activity, net photosynthetic rate (P-n), and protective enzyme activities under drought stress. The experiment included three conditions: Drought (D), Drought + Biochar (9 t hm(-2), DB), and CK (normal management). The results showed that biochar could significantly retard the loss of soil efficient moisture and effectively change the chlorophyll fluorescence parameters, including reducing the initial fluorescence (F-o) and J points to the relative variable fluorescence (V-j), while increasing the maximal photochemistry efficiency of photosystem (PS) II (F-v/F-m), the efficiency that a trapped electron can move further ahead of Q(A) (psi(o)), and the photosynthetic performance index (PIABS), thus effectively protecting PS II from damage. Biochar application also increased P-n and Rubisco activity. Compared with CK, the activity levels of superoxide dismutase (SOD) and peroxidase (POD) during drought stress increased rapidly to a peak and then began to decrease. When H2O2 was accumulated, there was clearance of CAT activity which was enhanced, accompanied by increased levels of a membrane lipid peroxidation product (MDA). However, MDA levels were always lower for DB than for D. By slowing the regulation of the photosynthetic physiological functions and cytoplasmic membrane peroxidation, the plants could significantly avoid serious damage and were more adaptable to drought stress.