Anticancer, Antibacterial and Hyperthermia Studies of a Caffeine-Based N-Heterocyclic Carbene Silver Complex Anchored on Magnetic Nanoparticles

摘要

Caffeine based N-heterocyclic carbene (NHC)-silver complex anchored on magnetic nanoparticles (MNP-Caff-NHC@Ag com- plex) has been prepared by covalent grafting of caffeine on the surface of chloro-functionalized Fe3O4 magnetic nanoparticles followed by complexation with silver (I) acetate. The MNP-Caff- NHC@Ag complex has been characterized by Fourier transform infrared (FT-IR) spectroscopy, energy-dispersive X-ray (EDX) spectroscopy, transmission electron microscopy (TEM), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and vibrat- ing sample magnetometer (VSM) analysis. The MNP-Caff- NHC@Ag complex displayed significant in vitro anticancer activity against human hepatocarcinoma HepG2 cell line and antibacterial activity against Escherichia coli (NCIM-2832), Staph- ylococcus aureus (NCIM-2654) and Bacillus cereus (NCIM-2703). The hyperthermia studies revealed that MNP-Caff-NHC@Ag complex achieved good therapeutic temperature (47 °C) under physiological safe range of field and frequency thereby forecasting potential applications as heating mediators in magnetic hyperthermia for selectively killing of cancer cells.