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Labeling accuracy and detection of DNA sequences originating from GMOs in meat products commercially available in Greece

机译:标记从GMO在希腊市售的肉类产品中源自转基因生物的DNA序列的标记精度和检测

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The aim of the present study was to assess labeling accuracy and detect food fraud practices as well as to detect DNA sequences originating from GMOs in meat products commercially available in Athens, Greece. A total of 139 different samples from 23 producers were included in the study. DNA was extracted from the samples and subjected to PCR aiming for species-specific sequences for the detection of pork, beef, chicken, turkey, donkey and horse meat as well as the screening elements P-35S, T-nos, CP4-epsps, pat and bar, for the detection of DNA originating from GMOs. In total, 72 products, i.e. 52% of the samples, were mislabeled and correct labeling was verified for the products originating from only 7 out of the 23 companies. This mislabeling may be attributed to lack of good manufacturing practices that resulted in cross-contamination in 31 cases, definite substitution in 3 cases and suspected substitutions in 37 cases. Donkey and horse meat as well as DNA sequences originating from GMOs were not detected.
机译:本研究的目的是评估标签的准确性,检测食品欺诈行为,以及检测希腊雅典市市售肉制品中源自转基因生物的DNA序列。该研究共包括来自23家生产商的139个不同样本。从样品中提取DNA,并进行PCR,目的是检测猪肉、牛肉、鸡肉、火鸡、驴和马肉的物种特异性序列,以及检测源自转基因生物的DNA的筛选元件P-35S、T-nos、CP4 EPSP、pat和bar。总共有72种产品(即52%的样品)被贴错标签,23家公司中只有7家公司的产品的标签正确。这种错误标签可能是由于缺乏良好的生产实践,导致31例交叉污染,3例明确替换,37例疑似替换。未检测到驴和马肉以及源自转基因生物的DNA序列。

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