Morphological study of bovine pregnant corpus luteum cells.

摘要

The corpus lutea obtained from 48 pregnant Holstein cows were examined morphologically to characterize intramitochondrial bodies (IMB) in the large luteum cells (LLC). The number and diameter of the IMB (200-900 nm) increased with the advance of pregnancy. Histochemically, the IMB reacted positively with protein and immunohistochemically with 11 alpha-hydroxyprogesterone-1-11 succinyl-bovine serum albumin. The cytoplasm of LLC contained small electron-dense granules, 150-200 nm in diameter with anorifice for secretion which reacted with relaxin. Small luteum cells had crystalline-like inclusions. It is suggested that these inclusion cells participate in the synthesis of steroids.