Quantification of fluorescent dyes in organ tissue samples via HPLC analysis

摘要

Highlights?Microspheres for blood perfusion measurements in pigs were extracted from brain, heart and kidney tissue samples.?Simultaneous quantification of four different microsphere types by extraction and separation of the encapsulated dyes.?Short run times of <3min and a sensitivity of ～1 sphere/g of tissue were achieved.?Microsphere concentrations in animals varied significantly, rendering microspheres problematic for perfusion measurements.AbstractThe determination of regional blood flowviathe accumulation of fluorescent microspheres is a concept regularly used in medical research. Typically, the microbeads get extracted from the tissue of interest and are then quantified by measuring the absorption or fluorescence of the incorporated dyes without further separation from the medium. However, in that case the absorption spectra of different dyes can overlap when used simul