Extraction of bioactive alkaloids from Melocactus zehntneri using supercritical fluid

摘要

The purpose of this study was to extract alkaloids from Melocactus zehntneri aerial parts using supercritical fluid extraction (SFE) and to evaluate their cytotoxic action. SFE process was compared to different classical low pressure solvent extraction processes in terms of global yield, extract profile and biological activity. Two specimens of M. zehntneri were evaluated: young and adult aerial parts. The extracts were analyzed by HPLC-UV/DAD as well as they were evaluated against HMVII and Trichomonas vaginalis. The results indicated a qualitative difference in chromatographic profile of the extracts. Finally, the results point out the potential of the extract obtained from aerial parts of young specimens (Y-RM) through SFE at 300 bar/55 degrees C against T. vaginalis and HMVII, presenting relevant information for development of new studies about cytotoxic. (C) 2017 Elsevier B.V. All rights reserved.