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Overexpression of GmGASA32 promoted soybean height by interacting with GmCDC25

机译:通过与GMCDC25进行交互,GMGASA32的过表达促进了大豆高度

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摘要

GmGASA is the GASA gibberellin regulated cysteine-rich protein family. The expression of GmGASA is up-regulated by gibberellin, which is the longest plant hormone in plants playing vital roles in plant development. However, very few reports explaining the direct regulation of downstream genes by GASA gene are available. In the current study, the GmGASA32, a member of the GASA family affecting soybean height was identified. In the early stage, preliminary verification of the response of GmGASA32 to gibberellin through phenotypic experiment was done. The promoter activity analysis confirmed that GmGASA32 was induced by gibberellin. Subcellular localization showed that GmGASA32-GFP fusion protein enriched in the nucleus after gibberellin treatment. In order to confirm the function of GmGASA32 in the nucleus, we confirmed that the GASA domain in the C terminal of GmGASA32 can interact with GmCDC25 (cell cycle-associated protein) through the bimolecular fluorescence complementation (BiFC) assay.
机译:GmGASA是由GASA赤霉素调节的富含半胱氨酸的蛋白质家族。GmGASA的表达受赤霉素的上调,赤霉素是植物中最长的植物激素,在植物发育中起着至关重要的作用。然而,很少有报道解释GASA基因对下游基因的直接调控。在目前的研究中,发现了影响大豆高度的GASA家族成员GmGASA32。在早期阶段,通过表型试验初步验证了GmGASA32对赤霉素的反应。启动子活性分析证实GmGASA32是由赤霉素诱导的。亚细胞定位显示,赤霉素处理后,GmGASA32-GFP融合蛋白在细胞核内富集。为了证实GmGASA32在细胞核中的功能,我们通过双分子荧光互补(BiFC)分析证实了GmGASA32 C端的GASA结构域可以与GmCDC25(细胞周期相关蛋白)相互作用。

著录项

  • 来源
    《Plant signaling & behavior》 |2021年第2期|共5页
  • 作者单位

    Jilin Agr Univ Coll Life Sci Engn Res Ctr Chinese Minist Educ Bioreactor &

    Pha Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Life Sci Engn Res Ctr Chinese Minist Educ Bioreactor &

    Pha Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Life Sci Engn Res Ctr Chinese Minist Educ Bioreactor &

    Pha Changchun 130118 Jilin Peoples R China;

    Jilin Agr Univ Coll Life Sci Engn Res Ctr Chinese Minist Educ Bioreactor &

    Pha Changchun 130118 Jilin Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物学;
  • 关键词

    GmGASA protein; gibberellin; nuclear localization; BiFC; GmCDC25;

    机译:Gmgasa蛋白质;吉布林素;核定位;BIFC;GMCDC25;

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