Lipid Profile and Total Phenolics of Cissus verticillata Seed and Leaf Extracts

摘要

Cissus verticillata (syn. Cissus sicyoides) leaf and seed samples were evaluated for their free radical scavenging activity, total phenolics, and lipid content. The DPPH assay was used to determine the free radical scavenging activity and the Folin-Ciocalteu method to determine total phenolics. Lipids were Soxhlet extracted, methylated and fatty acids identified by gas chro-matography-mass spectrometry. Leaves exhibited greater free radical scavenging activity (68.9 ± 0.8%) compared to seeds (10.3 ± 0.5%), with a total phenolic content of 6.28 and 1.78 mg gallic acid g-1 DW, respectively. Seeds, however, had a greater lipid content (14.4%) consisting mainly of linoleic acid (63.5%), palmitic acid (23.2%), and stearic acid (6.9%). The total lipid content of leaves was 0.9%, mainly consisting of linolenic acid (28.9%), palmitic acid (23.9%), linoleic acid (10.2%) and stearic acid (8.6%). Cissus verticillata seeds are currently underutilized and are a potential oil source rich in the essential omega-6 fatty acid, linoleic acid.