首页> 外文期刊>Oncology Research >Procaine Inhibits Proliferation and Migration and Promotes Cell Apoptosis in Osteosarcoma Cells by Upregulation of MicroRNA-133b
【24h】

Procaine Inhibits Proliferation and Migration and Promotes Cell Apoptosis in Osteosarcoma Cells by Upregulation of MicroRNA-133b

机译:普鲁卡因抑制细胞瘤细胞促进细胞凋亡,促进MicroRNA-133B的上调

获取原文
获取原文并翻译 | 示例
       

摘要

Procaine (PCA) is a conventional chemotherapeutic agent for osteosarcoma. Recent studies have proposed that the growth-inhibitory effect of PCA is through regulation of microRNAs (miRNAs). miR-133b has been proven to be a tumor suppressor in osteosarcoma, but whether it is involved in the antitumor effects of PCA on osteosarcoma has not been investigated. In this study, we aimed to explore the effects of PCA on osteosarcoma MG63 cells by regulation of miR-133b, as well as its underlying mechanisms. MG63 cells were treated with different concentrations of PCA, and cell viability, apoptosis, and miR-133b expression were then detected by MTT, flow cytometry, and qRT-PCR, respectively. Cells were then transfected with the miR-133b inhibitor and treated with 2 mu M PCA. Thereafter, cell viability, migration, and apoptosis were detected. Analysis of signaling pathways was detected by Western blot. Our results showed that PCA significantly inhibited cell viability and promoted apoptosis and the expression level of miR-133b in a dose-dependent manner (p < 0.05 or p < 0.01). Moreover, we observed that PCA+miR-133b inhibitor dramatically reversed the effects of PCA on cell viability, apoptosis, and migration (p < 0.05 or p < 0.01). In addition, PCA significantly decreased the levels of p/t-AKT (p308 or p473), p/t-ERK, and p/t-S6, whereas PCA + miR-133b inhibitor rescued these effects. Our results suggest that PCA inhibits proliferation and migration but promotes apoptosis in osteosarcoma cells by upregulation of miR-133b. These effects may be achieved by inactivation of the AKT/ERK pathways.
机译:None

著录项

  • 来源
    《Oncology Research》 |2017年第9期|共8页
  • 作者单位

    Jilin Univ Norman Bethune Hlth Sci Ctr Changchun Jilin Peoples R China;

    Jilin Univ China Japan Union Hosp Dept Orthoped 126 Xiantai St Changchun 130033 Jilin Peoples;

    Jilin Univ Norman Bethune Hlth Sci Ctr Changchun Jilin Peoples R China;

    Jilin Univ Norman Bethune Hlth Sci Ctr Changchun Jilin Peoples R China;

    Jilin Univ Norman Bethune Hlth Sci Ctr Changchun Jilin Peoples R China;

    Jilin Univ China Japan Union Hosp Dept Orthoped 126 Xiantai St Changchun 130033 Jilin Peoples;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 肿瘤学;
  • 关键词

    Procaine (PCA); Osteosarcoma; Proliferation; Migration; Apoptosis; MicroRNA-133b;

    机译:Procaine(PCA);骨肉瘤;增殖;迁移;细胞凋亡;microRNA-133B;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号