A label-free electrochemiluminescence aptasensor for carcinoembryonic antigen detection based on electrodeposited ZnS-CdS on MoS2 decorated electrode

摘要

In this work, a novel, simple and label-free electrochemiluminescence (ECL) strategy was designed for a sensitive carcinoembryonic antigen (CEA) assay on the basis of ZnS-CdS nanoparticle (NP)-decorated molybdenum disulfide (MoS2) nanocomposites. Layered-MoS2 nanocomposites with a large surface area were prepared with a facile hydrothermal method and used as an electrode matrix for subsequent ZnS-CdS NP electrodeposition. The ZnS-CdS NP decorated MoS2 electrode shows a higher ECL intensity than an electrode with only ZnS-CdS electrodeposition. For the fabrication of the aptasensor, chitosan was coated on a ZnS-CdS/MoS2/glass carbon electrode for CEA aptamer immobilization through a covalent coupling method. The ECL aptasensor for CEA detection exhibits a linear range from 0.05 to 20 ng mL(-1) (R = 0.9975) with a low detection limit of 0.031 ng mL(-1), and presents satisfactory specificity against some possible interfering proteins. Moreover, the method was successfully applied for CEA determination in human serum with recoveries of 80-111%, showing great promise for application in practice.