To explore the possibility of achieving a rapid and in situ detection of aflatoxin B-1 (AFB(1)), a portable biosensing instrument consisting of an impedance detector and a 3D-printed USB-compatible sensor chip was developed. In this study, we proposed an electrochemical biosensor based on screen-printed interdigitated microelectrodes (SPIMs) and a portable detector to achieve low-cost, highly selective and sensitive detection of AFB(1) in rice. Under optimized conditions, the immunosensor provided a detection limit of 5 ng mL(-1), which was below the allowable concentration. The total detection time including incubation was less than 1 h. The results obtained from this developed portable detection system were comparable to those from a commercial electrochemical station in the laboratory. Hence, the portable detector offers new tools for detection of a wide variety of analytes in clinical and environmental samples.
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机译：为了探索实现快速，原位检测黄曲霉毒素B-1（AFB（1））的可能性，开发了一种由阻抗检测器和3D打印的USB兼容传感器芯片组成的便携式生物传感仪器。在这项研究中，我们提出了一种基于丝网印刷叉指式微电极（SPIM）和便携式检测器的电化学生物传感器，以实现对水稻AFB（1）的低成本，高选择性和灵敏检测。在优化的条件下，免疫传感器的检测极限为5 ng mL（-1），低于允许的浓度。包括孵育在内的总检测时间少于1小时。从这种发达的便携式检测系统获得的结果与实验室中商用电化学工作站的结果相当。因此，便携式检测器提供了用于检测临床和环境样品中各种分析物的新工具。
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