In Vitro Propagation of Jojoba [Simmondsia chinensis (Link) Schneider] through Nodal Segments of Female Plants

摘要

Nodal segments from first, second and third nodes (from the tip of branch) of three mature female plants of jojoba [Simmondsia chinensis (Link) Schneider] were cultured on MS medium supplemented with different concentrations of BAP (1.0 to 5.0 mg U1).Number of days required for bud break ranged from 7-13 with 5.0 mg L1 BAP. Increasing BAP concentration from 1.0 to 4.0 mg L1, axilliary shoot bud proliferation/multiplication percentage increased. Second and third nodes exhibited better multiple shootformation (32.75%, 28.0%, respectively) as compared to the first node (15.52%) and the differences were significant. Shoot proliferation was successful at all the concentrations tested with a maximum number of 14 shoots per original explant within 12 weeks at 4.0 mg L1 BAP and maximum shoot length range. (1-3 cm) after 90 days was also observed on same medium. Rooting (42%) of micropropagated shoots was achieved in half MS solid medium containing 2.5 mg L-1 IBA.