Complementary use of in sacco and in vitro studies to investigate ruminal forage degradationin relation to gas production

摘要

Alfalfa hay (DM) degradation, was determined in sacco in three sheep for two periods and the results were adjusted according to 0rskov and Mc Donald (1979, J Agric Sci Camb, 92, 499-503). Compound samples of residues were taken at 0, 4, 12, 24 and 48h. Five sub-samples of each one (0.3 g) were incubated in vitro under anaerobic conditions at 39°C, for 24 h. The batch system used, consisted in 50 ml glass bottles that contained 30 ml filtered ruminal content (0.2 cm~2 mesh), connected to a 20 ml glass syringe. In control systems only ruminal fluid was incubated. Ruminal content was taken out of 3 fistulated sheep before morning feeding.