Study of the composition of the ruminal cellulolytic flora of bison and deer with rRNA-targeted oligonucleotide probes

摘要

The molecular phylogenetic framework provided by comparative rRNA sequencing has given a basis for describing the community structure of natural anaerobic ecosystems such as the rumen and for studying the ecology of microbial populations without the limitations imposed by traditional culture-based enumeration, pure culture-isolation and biochemical identification. The aim of this work was to investigate the qualitative and quantitative composition of the ruminal cellulolytic flora of bisons and deers by oligonucleotide probe hybridization.