High and Ultra Low Concentrations of Sodium Chloride Initiate their Action on Binding Sites of a Protein

摘要

There are clinical and experimental evidences which show that ultra low concentrations (ULC) of drugs used in homeopathy produce biological effects. ULCs differ from each other with respect to free water molecules or OH groups and H-bond strength of OH groups. The objective is to see whether ULCs of a drug, Natrum mur and its high concentration (θ) initiate their action on the binding sites of a protein, bovine serum albumin (BSA). Naim θ(500 m M NaCl solution in water) and its 2 potencies 30cH (100μM) and 200 cH (100μM) were prepared in the laboratory. The control was distilled water (55mM). The drug and control solutions were injected separately 20 times at 6 μl/injection every 2 min into a sample cell containing 7μM BSA at 25°C in the isothermal calorimetry (ITC) instrument. While Nat-m θ produced endothermic reaction, the potencies and the control produced exothermic reaction. ITC parameters binding constant (K), change in enthalpy (△H), entropy (△S) and free energy (△G) varied markedly from each other among the control and drug solutions. Control, Nat-m θ, 30cH and 2()()cH showed single site, sequential interaction in 3 sites, same in 3 sites and sequential interaction in 4 sites of BSA, respectively. It is concluded that the drug and the control initiate their action on binding sites of BSA. Nat-m θ and its potencies produced endothermic and exothermic reaction, respectively on BSA. Repetition of a dose produced increasing saturation.