Acidogenic mixed consortium isolated from soil of agricultural field: acid production behaviour and growth kinetics under the influence of pretreatment hydrolysate of rice straw (RS)

摘要

The present study was conducted to assess the possibilities of biofuel production through volatile fatty acid (VFA) platform using mixed consortia isolated from a natural source (soil of rice cultivation field). The acid production potential of the isolated acidogenic mixed consortium UACJUChEl, using pretreatment hydrolysate of rice straw as the carbohydrate source, was assessed. Growth kinetic parameters i.e. maximum specific growth rate (jumax) and substrate saturation constant (Ks) of UACJUChEl have been determined separately for both glucose and hydrolysate in the Reinforced Clostridial Medium (RCM). A summative type growth model has been observed to be valid when the combination of glucose and RS-hydrolysate (RSH) is used as the carbon sources. The summative model has been validated by successful comparison of model predictions with the experimental data. The presence of VFAs in the culture broth of the consortium grown on glucose, RS-hydrolysate and their combination (GlcRSH: 2:3; 1:4 (w/w)) has been established. The survival and growth of solventogenic Clostridium acetobutylicum in a growth medium amended with the effluent of these batch cultures of UACJUChEl have been ascertained. This indicates a strong potential of UACJUChEl in the hybridisation of the VFA platform and conventional sugar platform for the generation of butanol from RS.