Site-Specific DNA Cleavage on a Solid Support: A Method for Mismatch Detection

摘要

The growing insight into genetically caused diseases induced by single nucleotide polymorphisms (SNPs) calls for the development of analytical techniques to detect these SNPs. The techniques should be quick, reliable, and inexpensive. In recent years several strategies for genetic analyses were introduced, which are based on polymerase chain reactions (PCR),[1] enzymatic digestion,[2] electrochemistry,[3] or methods where target binding directly changes the fluorescence.[4] We now present a new concept for mismatch detection using chemical DNA cleavage on a solid support (Scheme 1): A single DNA strand, which carries a cleavage site at the central position and a detection tag at the 5-end, is immobilized on the solid support through the 3-end and hybridized with the target strand.