Novel approach to high-throughput determination of endocrine disruptors using recycled diatomaceous earth as a green sorbent phase for thin-film solid-phase microextraction combined with 96-well plate system

摘要

AbstractA sustainable approach to TF-SPME is presented using recycled diatomaceous earth, obtained from a beer purification process, as a green sorbent phase for the determination of bisphenol A (BPA), benzophenone (BzP), triclocarban (TCC), 4-methylbenzylidene camphor (4-MBC) and 2-ethylhexyl-p-methoxycinnamate (EHMC) in environmental water samples. TF-SPME was combined with a 96-well plate system allowing for high-throughput analysis due to the simultaneous extraction/desorption up to 96 samples. The proposed sorbent phase exhibited good stability in organic solvents, as well as satisfactory analytical performance. The optimized method consisted of 240?min of extraction at pH 6 with the addition of NaCl (15% w/v). A mixture of MeOH:ACN (50:50 v/v) was used for the desorption the analytes, using a time of 30?min. Limits of detection varied from 1?μg?L?1for BzP and TCC to 8?μg?L?1for the other analytes, and R2ranged from 0.9926 for 4-MBC to 0.9988 for BPA. This novel and straightforward approach offers an environmentally-friendly and very promising alternative for routine analysis. . The total sample preparation time per sample was approximately 2.8?min, which is a significant advantage when a large number of analytical run is required.Graphical abstractDisplay OmittedHighlights?Recycled diatomaceous earth is proposed as a novel and efficient sorbent phase for thin-film microextraction.?The recycled sorbent phase is environmentally friendly and easy to obtain.?Very satisfactory stability and analytical performance were achieved.?The methodology exhibited high-throughput and low solvent and sample consumption.]]>