Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome

摘要

Serum- and glucocorticoid-inducible kinase 1 in doxorubicin-induced nephrotic syndrome. Am J Physiol Renal Physiol 295: F1624-F1634, 2008. First published September 3, 2008; doi: 10.1152/ajprenal.00032.2008.-Doxorubicin-induced ne-phropathy leads to epithelial sodium channel (ENaC)-dependent volume retention and renal fibrosis. The aldosterone-sensitive serum- and glucocorticoid-inducible kinase SGK1 has been shown to participate in the stimulation of ENaC and to mediate renal fibrosis following mineralocorticoid and salt excess. The present study was performed to elucidate the role of SGK1 in the volume retention and fibrosis during nephrotic syndrome. To this end, doxorubicin (15 |xg/g body wt) was injected intravenously into gene-targeted mice lacking SGK1 (sgkl"^) and their wild-type littermates (sgkl+/+). Doxorubicin treatment resulted in heavy proteinuria (>100 mg protein/mg crea) in 15/44 of sgkl+/+ and 15/44 of sgkl~'~ mice leading to severe nephrotic syndrome with ascites, lipidemia, and hypoalbuminemia in both genotypes. Plasma aldosterone levels increased in nephrotic mice of both genotypes and was followed by increased SGK1 protein expression in sgkl+/+ mice. Urinary sodium excretion reached sign-ficantly lower values in sgkl+/+ mice (15 +- 5 |xmol/mg crea) than in sgkl~x~ mice beta5 +- 5 |xmol/mg crea) and was associated with a significantly higher body weight gain in sgkl+/+ compared with sgkl~y~ mice (+6.6 +- 0.7 vs. +4.1 +- 0.8 g). During the course of nephrotic syndrome, serum urea concentrations increased significantly faster in sgkl~y~ mice than in sgkl+/+ mice leading to uremia and a reduced median survival in sgkl~x~ mice (29 vs. 40 days in sgkl+/+ mice). In conclusion, gene-targeted mice lacking SGK1 showed blunted volume retention, yet were not protected against renal fibrosis during experimental nephrotic syndrome