Role of calcium-independent phospholipase A2 in complement-mediated glomerular epithelial cell injury

摘要

Previous studies have demonstrated that assembly of C5b-9 in cultured GEC is linked to the activation of CPLA2 and that glomeruli of rats with PHN demonstrate increased cPLA2 activity compared with control glomeruli (23). Activation of CPLA2 leads to various cellular effects. First, in GEC, activation of cPLA2 perturbs the membrane of the ER and contributes to induction of ER stress. Preconditioning of rats with compounds that induce ER stress can limit proteinuria in PHN (22). Second, activation of cPLA2 may contribute directly to the exacerbation of C5b-9-induced GEC injury (18, 23, 37). Finally, the AA mobilized by cPLA2 is metabolized by COX enzymes (44) to bioactive eicosanoids.