Development and characterization of Thy*IkappaBalpha-SI transgenic mice.

摘要

We developed and characterized a new transgenic model where NF-kappaB activity is inhibited only in mature neurons. Transgenic mouse strain Thy*IkappaBalpha-SI was created using trans-dominant super inhibitor NF-kappaB (IkappaBalpha-SI), which is a multimutant form of IkappaBalpha inhibitory protein cloned into specific neutral Thy-1.2 cassette. Detailed molecular analysis showed that the transgene and its product (IkappaBalpha-SI protein) are expressed in the nervous system of transgenic mice. In situ hybridization showed that Thy*IkappaBalpha-SI in the nervous system is expressed exclusively in neurons. The developed model provides wide opportunities for studying functional role of NF-kappaB in mature neurons in the central and peripheral nervous system in vivo.