Biohydrogen production in cassava processing wastewater using microbial consortia: Process optimization and kinetic analysis of the microbial community

摘要

Biohydrogen production was evaluated using cassava processing wastewater (CPW) and two microbial consortia (Vir and Gal) from different Brazilian environments. The biohydrogen production was optimized using a BoxBehnken design (T, pH, C/N, and % v/v inoculum). Maximum yields were obtained with hydrolyzed substrate: 4.12 and 3.80 mol H-2 / for Vir and Gal, respectively. Similarly, the kinetic parameters mu, k, and q were higher with hydrolyzed CPW in both consortia. The molecular analysis of the consortia through Illumina high throughput sequencing showed the presence of bacteria from the families Porphyromonadaceae, Clostridiaceae, Ruminococcaceae, and Enterococcaceae. The relative abundance of microbial families varies as fermentation progresses. In both consortia, Clostridiaceae reached the maximum relative abundance in the media between 16 and 24 h, interval in which approximately 90% of the biohydrogen is generated.